The Workshop on e-Business (WeB) is a prominent annual one-day event dedicated to advancing research focused on e-Business and e-Commerce. It is held in conjunction with the International Conference on Information Systems (ICIS) and organized by the Association for Information Systems (AIS). The theme for WeB 2025 is "E-business Transformation Facing AI-induced Uncertainties." This workshop aims to foster open dialogue and collaboration by bringing together researchers and industry leaders to share cutting-edge research, explore new ideas, reflect on successes and challenges, and collaboratively shape the future of e-Business in an era of rapid AI-driven change.